Interaction of Cycloserine
with the enzyme D-aminoacid aminotransferase
The antibiotic Cycloserine (antibiotic used to treat TB) belongs to the group of antibiotics which interferes with the cell wall synthesis of the bacteria by inhibiting biosynthetic enzymes involved in cell wall formation. Cycloserine interacts with D-aminoacid aminotransferase.
PDB file 2DAA has 3D coordinates of the complex containing D-aminoacid aminotransferase enzyme inactivated by Cycloserine Pyridoxal 5' phosphate which has bound to the active sites of both the chains A and B of the enzyme.
There are two identical chains of the enzyme i.e. A and B.
Chain A has 277 residues.
1 GYTLWNDQIV KDEEVKIDKE DRGYQFGDGV YEVVKVYNGE MFTVNEHIDR
51 LYASAEKIRI TIPYTKDKFH QLLHELVEKN ELNTGHIYFQ VTRGTSPRAH
101 QFPENTVKPV IIGYTKENPR PLENLEKGVK ATFVEDIRWL RCDIKSLNLL
151 GAVLAKQEAH EKGCYEAILH RNNTVTEGSS SNVFGIKDGI LYTHPANNMI
201 LKGITRDVVI ACANEINMPV KEIPFTTHEA LKMDELFVTS TTSEITPVIE
251 IDGKLIRDGK VGEWTRKLQK QFETKIP ......
Chain B is identical to chain A.
Click here to see the binding of SCP (Cycloserine Pyridoxal 5' phosphate) to the enzyme
(Fig. 1: Chimera rendered)
Click here to see the structure of SCP extracted from the enzyme
(Fig. 2: Chimera rendered)
Click here to look into the active site of chain B where a SCP molecule is bound
(Fig. 3: Chimera rendered)
